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Millipore Anti-Pd-L1 Antibody/Cd274 Is An Antibody Against Pd-L1/Cd274 For Use In Western Blotting & Flow Cytometry. - Mill (Additional S&H or Hazmat Fees May Apply)
Programmed cell death 1 ligand 1 (PD-L1/CD274) is a B7 related protein belonging to the BTN/MOG family. PDL-1/CD274 is essential for T-cell proliferation and production of IL10 and IFNG. PD-L1/CD274 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PD-L1/CD274 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PD-L1/CD274 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-PD-L1 Antibody/CD274 is an antibody against PD-L1/CD274 for use in western blotting & flow cytometry.
Key Applications
Western Blotting
Flow Cytometry
Application Notes
Western Blotting Analysis: 2 µg/mL from a representative lot detected PD-L1/CD274 in 10 µg of K562 cell lysate.
Flow Cytometry Analysis: 0.25 µg from a representative lot detected PD-L1/CD274 in 1X10E6 K562 cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the extracellular domain of human PD-L1/CD274.
Epitope
This antibody recognizes the Ig-like C2-type domain in the extracellular domain of PD-L1/CD274.
Concentration
1 mg/mL
Host
Rabbit
Specificity
This antibody should recognize isoforms 1 and 2 based upon immunogen design.
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Rhesus Macaque based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_054862
Gene Symbol
CD274
B7H1
PDCD1L1
PDCD1LG1
PDL1
Purification Method
Affinity Purfied
UniProt Number
Q9NZQ7
Molecular Weight
~55 kDa and ~33 kDa observed. Uniprot describes three isoforms at 33 kDa, 20 kDa and 21 kDa. This antibody contains 4 known glyscosylation sytes. An unglycosylated and glycosylated/membrane form may be observed at 34 kDa and 55 kDa, respectively.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected PD-L1/CD274 in 10 µg of Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
