Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Virus | IF, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABF1820-25UL |
| Description | Anti-Influenza A nucleoprotein |
| Alternate Names |
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| Background Information | Nucleoprotein (UniProt: Q9WLG4; also known as Nucleocapsid protein, Protein N) is encoded by the NP gene in Influenza A virus (strain A/Victoria/3/1975 H3N2). The genomes of all negative-strand RNA viruses are coated by the viral nucleoprotein. Together with the viral RNA-dependent RNA polymerase, these ribonucleoprotein complexes form the minimal functional unit for all viral transcription and replication. Influenza A virus has a negative-sense RNA genome that encodes a single-strand RNA-binding nucleoprotein. The primary function of the nucleoprotein is to encapsidate the virus genome for the purposes of RNA transcription, replication, and packaging. It is considered to be a critical factor in the viral infectious cycle in switching influenza virus RNA synthesis from transcription mode to replication mode. It also functions as a key adapter molecule between virus and host cell processes. It achieves this through its ability to interact with a wide variety of viral and cellular macromolecules, including RNA, itself, two subunits of the viral RNA-dependent RNA polymerase and the viral matrix protein. Nuceloprotein can also interact with cellular polypeptides, including actin, components of the nuclear import and export apparatus and a nuclear RNA helicase. Influenza A nucleoprotein has an unconventional nuclear localization signal (NLS) (aa 1-18) and a bipartite NLS (aa 1980216). (Ref.: Portela, A., and Digard, P. (2002). J. Gen. Virol. 83(4); 723-34; de Lucas, S., et Al. (2010). J. Virol. 84(15); 7603-7612). |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antiserum in 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Influenza A nucleoprotein, Cat. No. ABF1820, is a rabbit polyclonal antibody that detects influenza virus nucleoprotein and has been tested for use in Immunofluorescence and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A 1:3,000 dilution from a representative lot detected Influenza A nucleoprotein in A549 cells infected with A/PR/8/34 strain (Courtesy of Dr. Amelia Nieto at CNB). Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Influenza A nucleoprotein in A549 cells were infected with A/PR/8/34 strain (Courtesy of Dr. Amelia Nieto at CNB). Western Blotting Analysis: A representative lot detected Influenza A nucleoprotein in Western Blotting applications (Jorba, N., et. Al. (2009). PLoS Pathog. 5(5):e1000462; Lukarsk, M., et. Al. (2017). Nature. 541(7635):117-121; Tripathi, S., et. Al. (2015). Cell Host Microbe. 18(6):723-35). Immunofluorescence Analysis: A representative lot detected Influenza A nucleoprotein in Immunofluorescence applications (Marcos-Villar, L., et. Al. (2018). Sci Rep. 8(1):1230; Rodriguez-Fandsen, A., et. Al. (2016). Sci Rep. 6:20744). |
| Biological Information | |
|---|---|
| Immunogen | Hig-tagged full length recombinant nucleoprotein from Influenza A virus. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects influenza A virus nucleoprotein in virus infected cells. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Influenza A Virus. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~56 kDa observed; 56.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A549 cells infected with A/Victoria/3/75 strain of influenza virus. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Influenza A nucleoprotein in A549 cells infected with A/Victoria/3/75 strain of influenza virus. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |