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Millipore Anti-Peroxiredoxin-2 Antibody Is An Antibody Against Peroxiredoxin-2 For Use In Western Blotting & Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
The protein called Peroxiredoxin-2, or in older literature Thiol-specific antioxidant protein, TSA, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, Natural killer cell-enhancing factor B, PRP, or NKEF-B and encoded by the human gene PRDX2/NKEFB/TDPX1 plays a role in controlling the oxidation state of the cell by reducing peroxides via the Thioredoxin system. Peroxides are produced during normal metabolism and if not reduced, their built up can lead to oxidative stress and cell death. Interestingly Peroxirdoxin-2 can be inactivated itself by oxidative stress on Cys-51 thus its levels are tightly controlled. Peroxiredoxin-2 is particularly important in neurons where it acts as an anti-apoptotic factor but the protein is widely expressed and localizes to the cytoplasm in most cells. Because Peroxiredoxin-2 mediates H2O2 signaling it is considered an anti-aging and anti-cancer protein, but surprisingly recent research in yeast indicates that Peroxiredoxin-2 potentiates H202 levels and signaling by specifically inhibiting other Thioredoxin enzymes via their oxidation. Thus Peroxiredoxin-2 can both inhibit and promote peroxide metabolism depending upon the cellular microenvironment and cell systems being studied.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum without preservatives.
Quality Level
MQ100
Applications
Application
Anti-Peroxiredoxin-2 antibody is an antibody against Peroxiredoxin-2 for use in western blotting & ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Peroxiredoxin-2 in 10 µg of human brain and rat hippocampus tissue lysate.
Immunocytochemistry Analysis: A representative lot detected Peroxiredoxin-2 in COS cells (Prof. Mustapha Oulad, IGBMC, France.).
Biological Information
Immunogen
Ovalbumin-conjugated linear peptide corresponding to the C-terminus of mouse Peroxiredoxin-2.
Epitope
C-terminus
Host
Rabbit
Species Reactivity
Mouse
Human
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_035693
Gene Symbol
Prdx2
Tdpx1
Tpx
Purification Method
Unpurified
UniProt Number
Q61171
Molecular Weight
~22 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Peroxiredoxin-2 in 10 µg of mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
