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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1346 |
| Description | Anti-SHIP1 Antibody |
| Alternate Names |
|
| Background Information | Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (EC 3.1.3.86; UniProt Q92835; also known as hp51CN, Inositol polyphosphate-5-phosphatase of 145 kDa, p150Ship, SH2 domain-containing inositol 5-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, SIP-145) is encoded by the INPP5D (also known as SHIP, SHIP1) gene (Gene ID 3635) in human. SHIP1 and SHIP2 are related phosphatases that play important roles in modulating cell signaling by regulating the phosphorylation state of phosphatidylinositol phosphates (PIPs). While SHIP1 and SHIP2 share a high degree of amino acid conservation, they differ significantly in their cellular expression and receptor recruitment. SHIP1 is primarily confined to cells of the hematopoietic lineage, although it is also expressed by embryonic and mesenchymal stem cells (ESCs and MSCs). SHIP2 is ubiquitously expressed in all cell and tissue types, with especially high levels found in the heart, skeletal muscle, and placenta. SHIP1 functions as s negative regulator in immunoreceptor signaling, in hematopoietic progenitor cell proliferation/survival, and as an inducer of cellular apoptosis. Humna SHIP1 consists of an N-terminal SH2 domain (a.a. 5-101) that mediates interactions with a large number of intracellular signaling proteins. SHIP1 also contains three SH3-interacting regions (a.a. 124-129, 969-974, 1040-1051) characterized by the presence of the PxxP motifs and two NPXY motifs (a.a. 912-915 & 1019-1022) that interact with SH2 domain-containing proteins. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-SHIP1 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of SHIP1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SHIP1 in human lymphoma tissue, as well as in normal tonsil and kidney tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human SHIP1. |
| Epitope | Near C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Immunogen sequence is present in all three spliced isoforms of human SHIP1 reported by UniProt (Q92835). |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~145 kDa observed. Target band size appears larger than the calculated molecular weights of 133.63 kDa (isoform 1), 133.2 kDa (isoform 2), 109.3 kDa (isoform 3) due to posttranslational phosphorylation. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Ramos cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected SHIP1 in 10 µg of Ramos cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |