Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1388-25UG |
| Description | Anti-MUL1 |
| Alternate Names |
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| Background Information | Mitochondrial ubiquitin ligase activator of NFKB 1 (UniProt: Q969V5; also known as E3 SUMO-protein ligase MUL1, E3 ubiquitin-protein ligase MUL1, Growth inhibition and death E3 ligase, Mitochondrial-anchored protein ligase, MAPL, MAPL, Putative NF-kappa-B-activating protein 266, RING finger protein 218) is encoded by the MUL1 (also known as C1orf166, GIDE, MAPL, MULAN, RNF218) gene (GeneID: 79594) in human. MUL1 is a multi-pass membrane protein located on the outer mitochondrial membrane. It plays a role in the control of mitochondrial morphology and also promotes mitochondrial fragmentation and influences mitochondrial localization. MUL1 displays a weak E3 ubiquitin-protein ligase activity. It contains a RING-type zinc finger domain (aa 302-340), which is required for its E3 ligase activity. MUL1 can ubiquitinate Akt1 preferentially at Lys-284 involving Lys-48-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation. When over-expressed, MUL1 is reported to inhibit cell growth by activating JNK through MAP3K7/TAK1 and inducing caspase-dependent apoptosis. MUL1 is ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-MUL1, Cat. No. ABN1388, is a highly specific rabbit polyclonal antibody that targets Mitochondrial ubiquitin ligase activator of NFKB 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution of this antibody detected MUL1 in human cerebellum, human skeletal muscle myocytes, and human cerebral cortex. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linenar peptide corresponding to 12 amino acids from the C-terminal region of human Mitochondrial ubiquitin ligase activator of NFKB 1 (MUL1). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Mitochondrial ubiquitin ligase activator of NFKB 1 in Human skeletal muscle tissue. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 39 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human skeletal muscle tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected MUL1 in 10 µg of human skeletal muscle tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |