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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1445 |
| Replaces | 07-466 |
| Description | Anti-PPAR-gamma Antibody |
| Alternate Names |
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| Background Information | Peroxisome proliferator-activated receptor gamma (UniProt P37231; also known as Nuclear receptor subfamily 1 group C member 3, PPAR-gamma, PPARgamma) is encoded by the PPARG (also known as GLM1, NR1C3) gene (Gene ID 5468) in human. The peroxisome proliferator-activated receptors (PPARs) are nuclear receptor proteins that function as transcription factors. PPARs target genes play essential roles in the regulation of cellular differentiation, development, metabolism (carbohydrate, lipid, protein), and tumorigenesis. Three types of PPARs exist, namely alpha, delta (beta), and gamma. In addition, alternative splicings produce three PPAR-gamma isoforms. All PPARs heterodimerize with retinoid X receptor (RXR) and bind peroxisome proliferator hormone response elements (PPREs; AGGTCANAGGTCA) in the promoter region of target genes. PPARγ is activated by prostaglandin PGJ2 and certain members of the 5-HETE family of arachidonic acid metabolites, including 5-oxo-15(S)-HETE and 5-oxo-ETE. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PPAR-gamma Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PPAR-gamma. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in HUVECs, A431, HeLa, HEK293, and NIH/3T3 cells.. Western Blotting Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in 10 µg of human skeletal muscle tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human PPAR-gamma. |
| Epitope | Internal (N-terminal half). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody targets an internal sequence present in all spliced isoforms of human and murine PPAR-gamma reported by UniProt (P37231 and P37238). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Canine, Monkey, Feline, Porcine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~55/60 kDa observed. 54.68 kDa (human PPARgamma1(wt); isoform 1), 57.62 kDa (human isoform 2), 21.58 kDa (human PPARgamma1(tr); isoform 3), 54.51 kDa (mouse isoform 1), 57.60 kDa (mouse isoform 2) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected PPAR-gamma in 10 µg of MCF-7 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |