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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1446 |
| Description | Anti-GPRIN1 |
| Alternate Names |
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| Background Information | G protein-regulated inducer of neurite outgrowth 1 (UniProt Q7Z2K8; also known as GRIN1) is encoded by the GPRIN1 (also known as KIAA1893) gene (Gene ID 114787) in human. Originally identified as a binding protein for guanosine 5-3-O-(thio)triphosphate-bound G(z)alphaz, G protein-regulated inducer of neurite outgrowth 1 (GPRIN1) is a signaling molecule that functions as a downstream target of G(o)alpha. When co-expressed with constitutively active form of G(o)alpha, GPRIN1 is demonstrated to stimulate neurite extensions in Neuro2a cells. Gprin1 expression in mice is limited to the nervous system at all developmental stages, occurring within the entire embryonic mantle zones and becoming restricted to sets of nuclei at postnatal to adult stages. Gprin1 immunoreactivity is found colocalized with G(o)alpha at neuronal dendrites and axons, while being undetected in glial cells. Studies conducted in rat reveals that 7 nicotinic receptor ( 7) significantly reduces axon growth in hippocampal neurons by coupling to Gprin1, which targets it to the growth cone. RNAi-mediated Gprin1 knockdown abolishes the localization and calcium signaling of 7 at the growth cone. The 7/Gprin1 interaction appears intimately linked to a G(o)alpha, growth-associated protein 43, and CDC42 cytoskeletal regulatory pathway within the developing axon. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GPRIN1 Antibody, Cat. No. ABN1446, is a highly specific rabbit polyclonal antibody that targets GPRIN1 and has been tested in Immunohistochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GPRIN1 in human prostate cancer, cerebral cortex and pancreas tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to an internal sequence of human GPRIN1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody targets an internal sequence present in both spliced isoforms of human GPRIN1 protein reported by UniProt (Q7Z2K8). |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~105 kDa observed. 102.4 kDa (isoform 1) and 80.95 kDa (isoform 2) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HT-29 cell lysate. Western Blotting Analysis: A 1:20,000 dilution of this antibody detected GPRIN1 in 10 µg of HT-29 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |