Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1449 |
| Description | Anti-CXCR-4 |
| Alternate Names |
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| Background Information | C-X-C chemokine receptor type 4 (UniProt: P61073; also known as CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-1 receptor, CD184) is encoded by the CXCR4 gene (Gene ID: 7852) in human. CXCR-4 is a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium levels and enhancing MAPK1/MAPK3 activation. CXCR-4 is expressed in numerous tissues and two isoforms have been reported that are generated by alternative splicing. Isoform 1 is the predominant form in all tissues tested. CXCR-4 is rapidly phosphorylated on serine and threonine residues in the C-terminal upon agonist stimulation. Phosphorylation at Ser324 and Ser325 leads to recruitment of ITCH (E3 ubiquitin-protein ligase), ubiquitination and protein degradation. Sulfation on Tyr21 is shown to be essential for efficient binding of CXCL12/SDF-1alpha and it promotes its dimerization. Tyr7 and Tyr12 are sulfated in a sequential manner following complete sulfation of Tyr21. CXCR-4 acts as a co-receptor for human HIV-1 virus isolates (CD4 being the primary receptor) and as a primary receptor for HIV-2 isolates. CXCR-4 can also bind bacterial lipopolysaccharide (LPS) and mediate LPS-induced inflammatory response, including TNF-alpha secretion by monocytes. Mutations in CXCR4 gene cause WHIM syndrome that is characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CXCR-4 Antibody, Cat. No. ABN1449, is a highly specific rabbit polyclonal antibody that targets human CXCR-4 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CXCR-4 in human bone marrow, human pancreas, human breast and human prostate tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human CXCR-4. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects CXCR-4 in human cells. It targets an epitope within 12 amino acids from the N-terminal region. Immunogen sequence is conserved between isoforms 1 and 2. Hence, it may react with both isoforms. |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~53 kDa observed; 39.75 kDa calculated. Calculated molecular weight is 40 kDa but we observed it larger at around ~50 kDa.Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Raji membrane extract. Western Blotting Analysis: A 1:500 dilution of this antibody detected CXCR-4 in 10 µg of Raji membrane extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |