| Description | |
|---|---|
| Catalogue Number | ABN1469-25UL |
| Description | Anti-Alpha-2-HS-Glycoprotein |
| Alternate Names |
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| Background Information | Alpha-2-HS-glycoprotein (UniProt: P02765; also known as Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2 Heremans-Schmid glycoprotein, AHSG) is encoded by the AHSG (also known as FETUA, PRO2743) gene (Gene ID: 197) in human. Alpha-2-HS-glycoprotein is a serum protein of the cystatin superfamily that is synthesized by hepatocytes and is considered as a negative acute-phase reactant. It is highly expressed in various fetal tissues, but in adults it is mainly expressed in the liver. It is synthesized with a signal peptide (aa 1-18) and a connective propeptide (aa 301-340) and is subsequently cleaved into 2 chains known as Alpha-2-HS-glycoprotein chain A and Alpha-2-HS-glycoprotein chain B. The mature product is involved in multiple normal biological and pathological functions. It promotes possesses opsonic properties and is shown to be involved in endocytosis, brain development, bone resorption, regulation of insulin activity and hepatocyte growth factor (HGF) activity. Alpha-2-HS-glycoprotein also serves as a carrier protein and serves as a carrier for otherwise insoluble calcium phosphate and can act as a potent inhibitor of pathological vascular calcification. Higher levels of Alpha-2-HS-glycoprotein have been reported in gestational and type II diabetes. It is also present at higher levels in patients with endometriosis. Lower levels of Alpha-2-HS-glycoprotein are present in cancer subjects. (Ref.: Kalabay, L., et Al. (2002). Eur. J. Endocrinol. 147(2); 243-248; Sindhu, S., et Al. (2016). BMC Immunol. 17; 33). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Alpha-2-HS-Glycoprotein Antibody, Cat. No. ABN1469, is a highly specific rabbit polyclonal antibody that targets Alpha-2-HS-Glycoprotein and has been tested for use Immunohistochemistry and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected Alpha-2-HS-Glycoprotein in human retina tissue lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Alpha-2-HS-Glycoprotein in human liver and human bone marrow tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 146 amino acids from human Alpha-2-HS-glycoprotein. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects human Alpha-2-HS-glycoprotein. It targets an epitope with in the N-terminal half. |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~55 kDa observed; 39.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human serum. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Alpha-2-HS-Glycoprotein in human serum. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 µL |