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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1488 |
| Description | Anti-TIAM-1 |
| Alternate Names |
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| Background Information | T-lymphoma invasion and metastasis-inducing protein 1 (UniProt Q13009; also known as TIAM-1) is encoded by the TIAM1 gene (Gene ID 7074) in human. TIAM-1 is a ubiquitously expressed guanine nucleotide exchange factor (GEF) that specifically activates the Rho-like GTPase Rac1 in response to growth factors and cell-substrate interaction. TIAM-1 associates with GTP-bound Ras and functions as an effector that mediates Rac activation by Ras. TIAM-1 promotes the formation of adherens junctions and the induction of an epithelial-like phenotype in mesenchymal cells, and is essential for the formation and maintenance of cadherin-based adhesions. Tiam1-knockdown in mice protects against Ras-induced skin carcinogenesis as a result of increased apoptosis and reduced proliferation/growth of keratinocytes. However, the fewer skin tumors developed progress more frequently to malignancy in Tiam1-knockout mice, indicating that loss of Tiam1 stimulates malignant conversion. Cellular TIAM-1 is targeted for proteasome-dependent degradation by the SCFbTrCP ubiquitin ligase in cooperation with casein kinase 1 and the MEK/ERK pathway upon mitogenic stimulation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mm NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TIAM-1 Antibody, Cat. No. ABN1488, is a highly specific rabbit polyclonal antibody, that targets TIAM-1 and has been tested in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human TIAM-1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody targets a C-terminal region of human and mouse TIAM-1 sequences reported by UniProt (Q13009 and Q60610). |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Human, Rhesus Macaque, Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~190 kDa observed. 177.4/170.4 kDa (human isoform 1/2) and 177.4 kDa (mouse) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Neuro-2a cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected TIAM-1 in 10 µg of Neuro-2a mouse neuroblastoma cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |