Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IH(P), IP, WB | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1662 |
| Description | Anti-STRA6 |
| Alternate Names |
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| Background Information | Stimulated by retinoic acid gene 6 protein (UniProt: O70491; also known as Retinoic acid-responsive protein, STRA6) is encoded by the Stra6 gene (Gene ID: 20897) in murine species. STRA6 is a multi-pass membrane protein that acts as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). It has one intramembrane and nine transmembrane helices in a dimeric assembly. It acts by removing retinol from RBP/RBP4 and transports it across the plasma membrane, where it can be metabolized. This mechanism does not depend on endocytosis. Binds to RBP/RBP4 with high affinity. Increases cellular retinol uptake from the retinol-RBP complex. STRA6 is widely expressed in the embryo and it in adults it is expressed choroid plexus and the brain microvascular cells that form the blood-organ barrier. It is also expressed in the retinal pigment epithelium, seminiferous epithelium in testis, the yolk sac, and in chorioallantoic placenta. Its expression in Sertoli cells depends on the stage of the spermatogenic cycle. STRA6 can be induced by retinoic acid and it is shown to be synergistically up-regulated by Wnt1 and retinoid in mammary epithelial cells. Mutations in Stra6 gene have been linked to the Matthew-Wood syndrome that is characterized by pulmonary agenesis/hypoplasia; microphthalmia/anophthalmia; and congenital cardiac, digestive, and urogenital malformations. (Ref.: Sadowski, S et al. (2017). Birth Defects Res. 109(4); 251-253; Chen, Y et al. (2016). Science 353 (6302), aad8266). |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-STRA6, Cat. No. ABN1662, is a highly specific rabbit polyclonal antibody that targets Stimulated by retinoic acid gene 6 protein (STRA6) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot detected STRA6 in 293T cells transfected with an empty vector or pCMVTag2b-Stra6 (Courtesy of Dr. Noa Noy). Western Blotting Analysis: A representative lot detected STRA6 in Western Blotting applications (Berry, D.C., et. al. (2012). Mol Cell Biol. 32(19):3851-9). Western Blotting Analysis: A representative lot detected STRA6 in 293T cells transfected with an empty vector or pCMVTag2b-Stra6 (Courtesy of Dr. Noa Noy). Flow Cytometry Analysis: A 1:100 dilution from a representative lot detected STRA6 in HT-29 cells. Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected STRA6 in mouse testis and human liver cancer tissues. |
| Biological Information | |
|---|---|
| Immunogen | His-tggged recombinant fragment corresponding to 109 amino acids from the C-terminal region of murine Stimulated by retinoic acid gene 6 (STRA6) protein. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects murine Stimulated by retinoic acid gene 6 protein (STRA6). it targets an epitope within 109 amino acids from the C-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~90 kDa observed; 73.77 kda calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH3T3/L1Adipocytes. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected STRA6 in NIH3T3/L1 Adipocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |