Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, IF, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1669 |
| Description | Anti-Anoctamin-1 (TMEM16A) |
| Alternate Names |
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| Background Information | Anoctamin-1 (UniProt: Q8BHY3; also known as Transmembrane protein 16A, TMEM16A) is encoded by the Ano1 (also known as Tmem16a) gene (Gene ID: 101772) in murine species. Anoctamin-1 is a multi-pass membrane protein that along with TMEMB16B subunit serves as the calcium-activated chloride channels (CaCC). Two isoforms of Anoctamin-1 have been reported that are produced by alternative splicing. It is located in the apical membranes of epithelial cells in exocrine glands and in trachea. Its airway epithelial expression is increased in asthmatics. Hence, its abundance and activity may contribute to mucus secretion and airway hyper-responsiveness in asthma subjects. Anoctamin-1 is reported to be absent from smooth muscle cells, but present in the interstitial cells of Cajal (ICC), the pacemaker cells that control smooth muscle contraction. It acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. ATP and calmodulin are shown to be essential for its activation and the channel activity is inhibited by CFTR protein and by chloride channel inhibitors such as niflumic acid and 4, 4 -diisothiocyanatostilbene-2,2-disulfonic acid (DIDS). Mice with defective Tmem16a gene display severe tracheomalacia with gaps in the tracheal cartilage rings along the entire length of the trachea and over 90% of mutants die within the first nine days of postnatal life. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Anoctamin-1 (TMEM16A), Cat. No. ABN1669, is a highly specific rabbit polyclonal antibody that targets Anoctamin-1 (Transmembrane protein 16A) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected Anoctamin-1 (TMEM16A) in 10 µg of SW-620 and PC3 cell lysates. Immunofluorescence Analysis: A representative lot detected Anoctamin-1 (TMEM16A) in Immunofluorescence applications (Huang, F., et. al. (2009). Proc Natl Acad Sci USA. 106(50):21413-8; Wang, H.C., et. al. (2015). Cell. 163(6):1348-59; Huang, F., et. al. (2012). Proc Natl Acad Sci USA. 109(40):16354-9). Immunocytochemistry Analysis: A representative lot detected Anoctamin-1 (TMEM16A) in Immunocytochemistry applications (Huang, F., et. al. (2009). Proc Natl Acad Sci USA. 106(50):21413-8). Western Blotting Analysis: A representative lot detected Anoctamin-1 (TMEM16A) in Western Blotting applications (Huang, F., et. al. (2009). Proc Natl Acad Sci USA. 106(50):21413-8). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 14 amino acids from the N-terminal region of murine Anoctamin-1 (TMEM16A). |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Anoctamin-1/TMEM16A in human and murine cells. it targets an epitope within 14 amino acids from the N-terminal end. May react with both isoforms 1 and 2 based on sequence homology. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~111 kDa observed; 110.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Anoctamin-1 (TMEM16A) in 10 µg of A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |