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Millipore Anti-Calcipressin-1 Antibody, Cat. No. Abn1676, Is A Highly Specific Rabbit Polyclonal Antibody That Targets Calcipressin-1 And Has Been Tested In Immunocytochemistry And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Myocyte-enriched calcineurin-interacting protein 1
MCIP1
Regulator of calcineurin 1
Background Information
Calcipressin-1 (Uniprot: Q6IN33; also known as Down syndrome critical region protein 1 homolog, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1, RCAN-1) is encoded by the Rcan1 (also known as Dscr1) gene (Gene ID: 266766) in rat. Calcipressin-1 is a cytoplasmic protein that directly binds to the catalytic domain of calcineurin and acts as its negative regulator. When phosphorylated at the conserved FLISPP motif its capacity to inhibit calcineurin is increased. This phosphorylation also controls the half-life of calcipressin-1 by accelerating its degradation. It is a calcium-responsive enzyme, which is reported to dephosphorylate the nuclear factor of activated T cells (NFAT), which promotes its nuclear translocation and links calcium signaling to transcriptional regulation. Calcipressin-1 displays a quick turnover with a half-life of less than 3 hours. However, a Ser to Ala mutation at Ser163 prolongs its half-life to more than 9 hours. (Ref.: Genesca, L et al. (2003). Biochem. J. 374(2); 567-575).
Product Information
Format
Serum
Presentation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Calcipressin-1 Antibody, Cat. No. ABN1676, is a highly specific rabbit polyclonal antibody that targets Calcipressin-1 and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected Calcipressin-1 in cultured rat sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119).
Immunocytochemistry Analysis: A representative lot detected Calcipressin-1 in sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119).
Biological Information
Immunogen
A linear peptide corresponding to 11 amino acids from the C-terminal half of rat Calcipressin-1.
Epitope
unknown
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects calcipressin-1 (RCAN-1) in human and rat cells. It targets an epitope with in 11 amino acids from the C-terminal half.
Isotype
IgG
Species Reactivity
Rat
Human
Species Reactivity Note
Human, Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_714946
Gene Symbol
Rcan1
Dscr1
Purification Method
Unpurified
UniProt Number
Q6IN33
Molecular Weight
22.95 kDa calculated.
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in SH-SY5Y cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Calcipressin-1 in SH-SY5Y cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
