Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | ICC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1676-25UL |
| Description | Anti-Calcipressin-1 |
| Alternate Names |
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| Background Information | Calcipressin-1 (Uniprot: Q6IN33; also known as Down syndrome critical region protein 1 homolog, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1, RCAN-1) is encoded by the Rcan1 (also known as Dscr1) gene (Gene ID: 266766) in rat. Calcipressin-1 is a cytoplasmic protein that directly binds to the catalytic domain of calcineurin and acts as its negative regulator. When phosphorylated at the conserved FLISPP motif its capacity to inhibit calcineurin is increased. This phosphorylation also controls the half-life of calcipressin-1 by accelerating its degradation. It is a calcium-responsive enzyme, which is reported to dephosphorylate the nuclear factor of activated T cells (NFAT), which promotes its nuclear translocation and links calcium signaling to transcriptional regulation. Calcipressin-1 displays a quick turnover with a half-life of less than 3 hours. However, a Ser to Ala mutation at Ser163 prolongs its half-life to more than 9 hours. (Ref.: Genesca, L et al. (2003). Biochem. J. 374(2); 567-575). |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Calcipressin-1 Antibody, Cat. No. ABN1676, is a highly specific rabbit polyclonal antibody that targets Calcipressin-1 and has been tested in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Calcipressin-1 in cultured rat sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119). Immunocytochemistry Analysis: A representative lot detected Calcipressin-1 in sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 11 amino acids from the C-terminal half of rat Calcipressin-1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects calcipressin-1 (RCAN-1) in human and rat cells. It targets an epitope with in 11 amino acids from the C-terminal half. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | 22.95 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in SH-SY5Y cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Calcipressin-1 in SH-SY5Y cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |