Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IH(P), IF, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1687-100UG |
| Description | Anti-TBR-2 |
| Alternate Names |
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| Background Information | Eomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. TBR-2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of TBR-2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of TBR-2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et Al. (1999). Mech. Dev. 84 (1-2); 133-138). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-TBR-2, Cat. No. ABN1687, is a highly specific rabbit polyclonal antibody that targets Eomesodermin homolog (Tbr2) and has been tested in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A representative lot detected TBR-2 in Immunofluorescence applications (Nelson, B.R., et. Al. (2013). J Neurosci. 33(21):9122-39; Hodge, R.D., et. Al. (2013). J Neurosci. 33(9):4165-80). Immunohistochemistry Analysis: A representative lot detected TBR-2 in Immunohistochemistry applications (Yoon, K.J., et. Al. (2008). Neuron. 58(4):519-31). Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TBR-2 in mouse hippocampus, embryonic mouse brain, and embryonic mouse gut tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | Conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of murine TBR-2. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects T-box brain protein 2 (TBR-2) in murine species. It targets an epitope with in 20 amino acids from the C-terminal region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~63 kDa observed; 74.80 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse E13-14 brain. Western Blotting Analysis: A 1:500 dilution of this antibody detected TBR-2 in mouse E13-14 brain. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |