| Description | |
|---|---|
| Catalogue Number | ABN1699 |
| Description | Anti-Prickle1 |
| Background Information | Prickle-like protein 1(UniProt: Q3U5C7) is encoded by the Prickle1 (also known as Pk1) gene (Gene ID: 106042) in murine species. Prickle Prickle-like Protein 1 is involved in the planar cell polarity (PCP) pathway that controls convergent extension during gastrulation and neural tube closure. PCP pathway comprises of multiple integral membrane proteins ((Frizzled, Flamingo/Stan/Celsr and Vangl/ Strabismus) and membrane-associated proteins (Dishevelled, Inversin, and Prickle). Prickle-like protein 1 is synthesized with a propeptide (aa 830-832) that is removed in mature protein. Prickle1 is expressed in distinct cell populations of the central nervous system and is a contributor to neuronal morphogenesis. Both Pk1 and Pk2 genes share three LIM (Lin 11, Isl-1 and Mec-3) and one PET domain (Prickle, Espinas and Testin) at the N-terminus of the protein and a CAAX prenylation motif at the C-terminus. Pk1 is shown to be expressed in the cortical subplate and is considered as an important site for early neuronal maturation. In the retinal tissue Pk1 expression is specific to neurons and is highly enriched in Starburst amarcine cells. Loss of Pk1 gene has been linked to early embryonic lethality. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Prickle1 , Cat. No. ABN1699, is a highly specific rabbit polyclonal antibody that targets Prickle-like protein 1 and has been tested in Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot detected Prickle1 in Western Blotting applications (Liu, C., et. al. (2014). Biol Open. 3(9):861-70). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged Recombinant Protein corresponding to the C-terminus of Mouse Prickle-like protein 1. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Prickle-like protein 1 in murine species. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Predicted to react with Human, Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~94 kDa observed; 94.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse retinal tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Prickle1 in mouse retinal tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |