Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IF, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1712 |
| Description | Anti-NRL (Neural Retina Leucine Zipper) |
| Alternate Names |
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| Background Information | Neural retina-specific leucine zipper protein (UniProt: P54846; also known as NRL) is encoded by the Nrl gene (Gene ID: 18185) in murine species. NRL is a bZIP transcription factor of the MAF family that regulates the expression of several rod-specific genes, including RHO and phosphodiesterase 6B. NRL is reported to be present in the nuclei of developing and mature rods but not cones. It interacts with the homeodomain protein CRX and synergistically regulates rhodopsin expression. It is also reported to interact with FIZ1 and this interaction can repress transactivation. It consists of a minimal transactivation domain (aa 30-93), a basic motif (aa 159-185), and a leucine-zipper (aa 187-208). The minimal transactivation domain (MTD) is conserved across the MAF family and it may activate transcription by recruiting TBP and associated factors at the promoters of target genes. In developing mouse retina, Nrl expression is reported to precede the appearance of known markers for differentiated rods. The transcriptional activity of NRL is modulated by sumoylation on RHO and NR2E3 promoters. (Ref.: Prabodha, K et al. (2001). J. Biol. Chem 276 (39): 36824-30). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NRL (Neural Retina Leucine Zipper), Cat. No. ABN1712, is a highly specific rabbit polyclonal antibody that targets Neural retina-specific leucine zipper protein and has been tested for use in Immunofluorescence and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A 1:100-1:1,000 dilution from a representative lot detected NRL (Neural Retina Leucine Zipper) in frozen mouse retina tissue (Courtesy of Dr. Tiansen Li at NIH). Western Blotting Analysis: A 1:1,000-1:2,000 dilution from a representative lot detected NRL (Neural Retina Leucine Zipper) in mouse retinal tissue lysate (Courtesy of Dr. Tiansen Li at NIH). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant protein fragment corresponding to 108 amino acids from the N-terminal half of murine neural retina-specific leucine zipper protein. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody recognizes murine neural retina-specific leucine zipper protein. It iargets an epitope within 108 amino acids from the N-terminal half. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~31 kDa observed; 26.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse retinal tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NRL (Neural Retina Leucine Zipper) in mouse retinal tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |