Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ICC, IF, IHC, WB | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN1734-25UL |
| Description | Anti-SNAT2 |
| Alternate Names |
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| Background Information | Sodium-coupled neutral amino acid transporter 2 (UniProt: Q9JHE5; also known as Amino acid transporter A2, Solute carrier family 38 member 2, System A amino acid transporter 2, System A transporter 1, System N amino acid transporter 2) is encoded by the Scl38a2 (also known as Ata2, Sa1, Sat2, Snat2) gene (Gene ID: 29642) in rat. Sodium-coupled neutral amino acid transporter 2 is a multi-pass membrane protein that functions as a sodium-dependent amino acid transporter and mediates the saturable, pH-sensitive and electrogenic cotransport of neutral amino acids and sodium ions with a stoichiometry of 1:1. It may also function in the transport of amino acids at the blood-brain barrier and in the supply of maternal nutrients to the fetus through the placenta. Its transport action is reported to be inhibited by N-methyl-D-glucamine and choline. It is enriched in the somatodendritic compartment of neurons and is also detected at the axonal shaft, but is excluded from the nerve terminal. It is also expressed in skeletal muscle and adipose tissue. Its levels are up-regulated upon amino acid deprivation that results from increased transcription and protein stabilization. |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-SNAT2, Cat. No. ABN1734, is a highly specific rabbit polyclonal antibody that targets Sodium-coupled neutral amino acid transporter 2 and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A 1:150 dilution from a representative lot detected SNAT2 in rat cerebral cortex and human cerebellum tissue sections. Immunocytochemistry Analysis: A representative lot detected SNAT2 in Immunocytochemistry applications (Grewal, S., et. Al. (2009). J Biol Chem. 284(17):11224-36). Western Blotting Analysis: A representative lot detected SNAT2 in Western Blotting applications (Yao, D., et. Al. (2000). J Biol Chem. 275(30):22790-7; Melone, M., et. Al. (2006). Neuroscience. 140(1):281-92; Grewal, S., et. Al. (2009). J Biol Chem. 284(17):11224-36). Immunohistochemistry Analysis: A representative lot detected SNAT2 in Immunohistochemistry applications (Melone, M., et. Al. (2006). Neuroscience. 140(1):281-92). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to the first 65 amino acids from TE N-terminal region of rat Sodium-coupled neutral amino acid transporter 2 (SNAT2). |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects sodium-coupled neutral amino acid transporter 2 (SNAT2) in rat brain. It targets an epitope within the first 65 amino acids. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Rat. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | 55.55 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunofluorescence in rat brain sections. Immunofluorescence Analysis: A 1:150 dilution of this antibody detected SNAT2 in rat brain tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |