Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | IF, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN2181-25UG |
| Description | Anti-NCAM-L1 |
| Alternate Names |
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| Background Information | Neural cell adhesion molecule L1-like protein (UniProt: P70232; also known as Cell adhesion molecule with homology to L1CAM, NCAM-L1, Chl1-like protein, Close homolog of L1) is encoded by the Chl1 (also known as Call) gene (Gene ID: in murine species. NCAM-L1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off in the mature form. It is expressed in neurons and glial cells in the brain and the spinal cord and has also been detected in the retina and the optic nerve. It is not detectable in mouse forebrain at E11 and becomes weakly detectable at E13 with highest detection at E18 to postnatal day 7. It is down-regulated at postnatal day 15 and is further reduced in four-week-old animals. NCAM-L1 is heavily N-glycosylated and contains N-linked oligosaccharides with a sulfated carbohydrate structure type HNK-1. NCAM-L1 contains an extracellular domain (aa 26-1083), a transmembrane domain (aa 1084-1104), and a cytoplasmic domain (aa 1105-1209). It also has six Ig-like C2 type domains and four fibronectin type -III domains. NCAM-L1 is an extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. It plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of interneurons and the efficacy of GABAergic synapses. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-NCAM-L1, Cat. No. ABN2181, is a highly specific rabbit polyclonal antibody that targets Neural cell adhesion molecule L1 and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected NCAM-L1 in Western Blotting applications (Schmidt, H., et. al. (2002). J Cell Biol. 159(3):489-98; Alberts, P., et. al. (2003). Mol Biol Cell. 14(10):4207-20). Immunoprecipitation Analysis: A representative lot immunoprecipitated NCAM-L1 in Immunoprecipitation applications (Patel, K., et. al. (1991). Hybridoma. 10(4):481-91). Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NCAM-L1 in crude membrane fractions of postnatal day 10 brain extracts (Courtesy of Fritz G. Rathjen from Max-Delbrück-Center, Berlin Germany). Immunofluorescence Analysis: A representative lot detected NCAM-L1 in Immunofluorescence applications (Ter-Avetisyan, G., et. al. (2014). J Neurosci. 34(3):737-47; Abe, P., et. al. (2015). J Neurosci. 35(38):13053-63). |
| Biological Information | |
|---|---|
| Immunogen | Purified full-length murine Neural cell adhesion molecule L1-like protein (NCAM-L1). |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects NCAM-L1 protein in mouse and rat. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A purified |
| UniProt Number |
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| Molecular Weight | ~220 kDa observed; 135.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Wild type & knock out P10 mouse brain extract crude membrane. Western Blotting Analysis: 2 µg/mL of this antibody detected NCAM-L1 in crude membrane extact from brain of Wild type, but not in NCAM-L1 knock out P10 mouse. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |