Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN2191 |
| Description | Anti-Calretinin |
| Alternate Names |
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| Background Information | Calretinin (UniProt: P47728; also known as CR) is encoded by the Calb2 gene (Gene ID: 117059) in rat. Calretinin belongs to the calbindin protein family and acts as a calcium-binding protein. It is expressed in both central and peripheral nervous system and is found abundantly in auditory neurons. It is involved in regulating cytosolic calcium ion concentrations and has multiple calcium-binding domains known as EF-hands. Its expression is shown to be regulated at the level of transcription by nuclear respiratory factor 1 (NRF-1) and E2F2 transcription factors. In rat striatum calretinin neurons form a distinct subset of medium-sized (7 20 µm), aspiny, GABAergic interneurons. Calretinin can also serve as an important differential diagnostic tool for cystic and solid ameloblastic tumors. (Ref.: Petryszyn, S., et al. (2017). J. Neural Transm. doi: 10.1007/s00702-017-1687; Kresoja-Rakic, J., et al. (2017). Front. Genet. doi: 10.3389/fgene.2017.00070). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Calretinin Antibody, Cat. No. ABN2191, is a highly specific rabbit polyclonal antibody that targets Calretinin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected Calretinin in rat brain tissue lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Calretinin in human cerebral cortex and rat cerebellum tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 99 amino acids from the internal region of rat Calretinin. |
| Epitope | domain of: |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Calretinin in human and rat cells. It targets an epitope within 99 amino acids from the internal region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~31 kDa observed; 31.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human fetal brain tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Calretinin in human fetal brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |