| Description | |
|---|---|
| Catalogue Number | ABN2246 |
| Description | Anti-PTP zeta/Phosphacan |
| Alternate Names |
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| Background Information | Receptor-type tyrosine-protein phosphatase zeta (UniProt: Q62656; also known as EC:3.1.3.48, R-PTP-zeta, 3F8 chondroitin sulfate proteoglycan, 3H1 keratan sulfate proteoglycan, Phosphacan) is encoded by the Ptprz1 (also known as Ptprz, Ptpz) gene (GeneID: 25613) in rat. It is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth and negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. It is required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. RPTPzeta has an extracellular domain (aa 25-1637), a transmembrane domain (aa 1638-1663) and a cytoplasmic domain (aa 1664-2316). It is produced with a signal peptide (aa 1-24) that is cleaved to generate the mature form. |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phosphacan, Cat. No. ABN2246 is a rabbit polyclonal antibody that has been tested for use in Immunohistochemistry (Paraffin) for the detection of Receptor-type tyrosine-protein phosphatase zeta. |
| Key Applications |
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| Application Notes | Immunohistochemistry Analysis: A representative lot detected PTP zeta/Phosphacan in E16 rat spinal cord (Milev, P., et. al. (1994). J Cell Biol. 127(6 Pt 1):1703-15), rat brain (Haunso, A., et. al. (1999). Brain Res. 834(1-2):219-22), and 21-day postnatal rat retina, 2-week postnatal rat cerebellum, E13 rat spinal cord (Milev, P., et. al. (1998). J Biol Chem. 273(12):6998-7005). |
| Biological Information | |
|---|---|
| Immunogen | Full length (aa 1-2316) purified Receptor-type tyrosine-protein phosphatase zeta (Phosphacan) isolated from 7-day fetal rat brain. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects PTP zeta/Phosphacan in rat brain tissue. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | 255.34 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in rat cerebral cortex and rat hippocampus tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PTP zeta/Phosphacan in rat cerebral cortex and rat hippocampus tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |