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Millipore Anti-Phosphacan, Cat. No. Abn2246 Is A Rabbit Polyclonal Antibody That Has Been Tested For Use In Immunohistochemistry (Paraffin) For The Detection Of Receptor-Type Tyrosine-Protein - Mill (Additional S&H or Hazmat Fees May Apply)
Receptor-type tyrosine-protein phosphatase zeta (UniProt: Q62656; also known as EC:3.1.3.48, R-PTP-zeta, 3F8 chondroitin sulfate proteoglycan, 3H1 keratan sulfate proteoglycan, Phosphacan) is encoded by the Ptprz1 (also known as Ptprz, Ptpz) gene (GeneID: 25613) in rat. It is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth and negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. It is required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. RPTPzeta has an extracellular domain (aa 25-1637), a transmembrane domain (aa 1638-1663) and a cytoplasmic domain (aa 1664-2316). It is produced with a signal peptide (aa 1-24) that is cleaved to generate the mature form.
Product Information
Format
Unpurified
Presentation
Rabbit polyclonal antiserum with 0.02% sodium azide.
Applications
Application
Anti-phosphacan, Cat. No. ABN2246 is a rabbit polyclonal antibody that has been tested for use in Immunohistochemistry (Paraffin) for the detection of Receptor-type tyrosine-protein phosphatase zeta.
Key Applications
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A representative lot detected PTP zeta/Phosphacan in E16 rat spinal cord (Milev, P., et. al. (1994). J Cell Biol. 127(6 Pt 1):1703-15), rat brain (Haunso, A., et. al. (1999). Brain Res. 834(1-2):219-22), and 21-day postnatal rat retina, 2-week postnatal rat cerebellum, E13 rat spinal cord (Milev, P., et. al. (1998). J Biol Chem. 273(12):6998-7005).
Biological Information
Immunogen
Full length (aa 1-2316) purified Receptor-type tyrosine-protein phosphatase zeta (Phosphacan) isolated from 7-day fetal rat brain.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody specifically detects PTP zeta/Phosphacan in rat brain tissue.
Isotype
IgG
Species Reactivity
Rat
Species Reactivity Note
Rat.
Antibody Type
Polyclonal Antibody
Gene Symbol
Ptprz1
Ptprz
Ptpz
Purification Method
Unpurified
UniProt Number
Q62656
Molecular Weight
255.34 kDa calculated.
Product Usage Statements
Quality Assurance
Evaluated by Immunohistochemistry (Paraffin) in rat cerebral cortex and rat hippocampus tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PTP zeta/Phosphacan in rat cerebral cortex and rat hippocampus tissue.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
