Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN2261-25UG |
| Description | Anti-GAS2L1 |
| Alternate Names |
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| Background Information | GAS2-like protein 1 (UniProt: Q99501-1; also known as GAS2-related protein on chromosome 22, Growth arrest-specific protein 2-like 1, GAS2L1) is encoded by the GAS2L1 (also known as GAR22) gene (Gene ID: 10634) in human. GAS2L1 belongs to the growth-arrest-specific 2 (GAS2) family, whose members mediate the crosstalk between filamentous actin (F-actin) and microtubules. Three members of this family, known as GAS2L1, GAS2L2, and GAS2L3, are shown to be differentially involved in mediating the crosstalk between F-actin and microtubules. GAS2L1 is expressed in testis and brain, GAS2L2 is exclusively expressed in skeletal muscle, and GAS2L3 is found in many different cell types. Although they all localize to actin and microtubules, only the exogenous expression of GAD2L1 and GAS2L2 affect microtubule stability, dynamics, and guidance along actin stress fibers. GAS2L1 is reported to associate with the proximal end of mature centrioles and participates in centriole dynamics and centrosome disjunction. Loss of GAS2L1 can inhibit centrosome disjunction in the G2 phase. On the other hand, overexpression of GAS2L1 can induce premature centrosome separation, which also requires its association with actin, microtubules, and the microtubule end-binding proteins. (Ref.: Stroud MJ et Al. (2014). J. Cell Sci. 127(12); 2672-2682; AU, FKC et Al. (2017). Dev. Cell 40(1); 81-94). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 10% glycerol. |
| Applications | |
|---|---|
| Application | Anti-GAS2L1, Cat. No. ABN2261, is a highly specific rabbit polyclonal antibody that targets GAS2-like protein 1 (Gas2L1) and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected GAS2L1 in Western Blotting applications (Au, F.K., et. Al. (2017). Dev Cell. 40(1):81-94). Immunoprecipitation Analysis: 1 µg from a representative lot detected GAS2L1 in U2OS cell lysate (Courtesy of Dr Robert Z. Qi and Dr. Franco K.C. Au at Hong Kong University of Science and Technology). Immunofluorescence Analysis: A representative lot detected GAS2L1 in Immunofluorescence applications (Au, F.K., et. Al. (2017). Dev Cell. 40(1):81-94). Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAS2L1 in lysate from HeLa cells, but not in lysate from GAS2L1 knockout cells (Courtesy of Dr Robert Z. Qi and Dr. Franco K.C. Au at Hong Kong University of Science and Technology). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 188 amino acids from the C-terminal region of human GAS2-Like protein 1. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody GAS2-like protein 1 (GAS2L1) in human cells. It targets an epitope with in 188 amino acids from the C-terminal half. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~78 kDa observed; 72.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa and GAS2L1 knockout lysate. Western Blotting Analysis: 0.9 µg/mL of this antibody detected GAS2-like protein 1 (Gas2L1) in HeLa cell lysate. It did not detect GAS2L1 in lysate from knockout cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |