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Millipore Detect Complex I-75kd Using This Rabbit Polyclonal Antibody, Anti-Complex I-75kd Antibody Validated For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Complex I-75kD (CI-75kD) is also called NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1). Complex I-75kD is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which transfers electrons from NADH to the respiratory chain, and is thought to be part of the minimal assembly required for catalysis. Complex I-75kD defects are thought to be a cause of Mitochondrial complex I deficiency (MT-C1D).
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Complex I-75kD using this rabbit polyclonal antibody, Anti-Complex I-75kD Antibody validated for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Complex I-75kD in 10 µg in human brain and human heart tissue lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Complex I-75kD in human kidney and human cardiac myocytes tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Complex I-75kD.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Mouse
Rat
Species Reactivity Note
Predicted to react with Bovine, Chimpanzee, Orangutan based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_004997
Gene Symbol
NDUFS1
Purification Method
Affinity Purfied
UniProt Number
P28331
Molecular Weight
~75 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Complex I-75kD in 10 µg of mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
