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Millipore This Anti-Phospho-Neurogranin (Ser36)/Neuromodulin (Ser41) Antibody Is Validated For Use In Western Blotting For The Detection Of Phospho-Neurogranin (Ser36)/Neuromodulin (Ser41). - Mill (Additional S&H or Hazmat Fees May Apply)
Neurogranin (Ng) (also named RC3, p17 or BICKS) is a small protein originally identified in rat brain and abundantly expressed in several telencephalic areas, such as the cerebral cortex, hippocampus, amygdala, and striatum. In neurons, it is found concentrated at dendritic spines where it participates in synaptic signaling events through the regulation of calmodulin (CaM). Neurogranin protein is a critical third messenger and substrate of PKC in the molecular cascade necessary for synaptic development and remodeling. Neurogranin (Ng) features an IQ motif that mediates its interaction with CaM and phosphatidic acid (PA). The interaction is controlled by phosphorylation at serine 36 of Neurogranin. Neurogranin is phosphorylated at serine 36 by PKC. Ser36-phosphorylated Neurogranin is unable to bind either CaM or PA. Neurogranin in localized in the cell bodies of neurons in the cortex and in the apical and basal dendrites of pyramidal neurons. Neurogranin is not found in dendrites and its expression is very low as well in Alzheimer’s disease patients.
Product Information
Format
Serum
Presentation
Rabbit Polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-phospho-Neurogranin (Ser36)/Neuromodulin (Ser41) Antibody is validated for use in Western Blotting for the detection of phospho-Neurogranin (Ser36)/Neuromodulin (Ser41).
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the IQ domain of Rat phospho-Neurogranin.
Epitope
IQ domain
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Phosphorylated Neurogranin and Neuromodulin
Species Reactivity
Mouse
Species Reactivity Note
Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_077054
Gene Symbol
Nrgn
neurogranin
Ng
Neurogranin
hng
RC3
Modifications
Phosphorylation
Purification Method
Unpurified
UniProt Number
Q04940
Molecular Weight
~43 kDa is phospho-Neuromodulin (GAP-43) and ~17 KD is phospho-Neurogranin. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:1000 dilution of this antibody detected phospho-Neurogranin (Ser36) / Neuromodulin (Ser41) in 10 µg of Mouse brain tissue lysate treate with magnisium (lane1) or PKC (lane2)
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
