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Millipore This Anti-Elfn1 Antibody Is Validated For Use In Western Blotting And Immunohistochemistry For The Detection Of Elfn1. - Mill (Additional S&H or Hazmat Fees May Apply)
Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1
Protein phosphatase 1 regulatory subunit 28
Background Information
ELFN1 is a postsynaptic protein that regulates circuit dynamics in the CNS by modulating the temporal dynamics of interneuron recruitment. ELFN1 is specifically expressed in excitatory synapses onto orens-lacunosum (OLM) interneurons and acts as a regulator of presynaptic release in directing of the pyramidal-OLM synapses. ELFN1 localizes to excitatory synapses on somatostatin containing OLM interneurons at the plasma membrane, and ELFN1 protein, expressed post-synaptically, can direct the elaboration of specific electrochemical properties of synapses between particular cell types in the hippocampus. ELFN1’s expression is found in distinct subsets of interneurons of the hippocampus and cortex, and also in discrete subsets of cells in the habenula, septum, globus pallidus, dorsal subiculum, amygdala and several other brain regions in the mouse. The protein is also expressed in diverse cell types, including local GABAergic interneurons as well as long-range projecting GABAergic and glutamatergic neurons.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ300
Applications
Application
This Anti-ELFN1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ELFN1.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ELFN1 in human thalamus and rat cerebral cortex tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human ELFN1 near the N-terminal, extracellular domain.
Epitope
Near N-terminal, extracellular domain
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Monkey, Primate, Chimpanzee, Baboon, Hamster based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001122108
Gene Symbol
ELFN1
PPP1R28
Purification Method
Affinity Purfied
UniProt Number
P0C7U0
Molecular Weight
~100 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ELFN1 in 10 µg of U2OS cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
