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Millipore This Anti-Amyloid-Like Protein 1 Antibody Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Amyloid-Like Protein 1. - Mill (Additional S&H or Hazmat Fees May Apply)
Amyloid-like protein 1 or APLP-1 is neuronal, membrane bound specific protein that likely has a role in post-synaptic function but most research has focused on its proteolytic fragments such as ALID1 which acts as a transcriptional activator through binding the co-activator APBB1. APLP-1 fragments also interact with various G-protein coupled receptor pathways and appear to have a role in synaptic maturation during cortical development. APLP-1 also appears to regulate neurite outgrowth through binding to components of the ECM such as heparin and collagen I. Finally a special fragment called C30 is a potent enhancer of neuronal apoptosis and is a caspase specific cleavage fragment that works internally inducing apoptosis. Thus APLP-1 has roles in apoptosis, cell adhesion, endocytosis, cell signaling and neurite extension as well as some post-synaptic function as it is found clustered at post-synaptic densities in normal cells.
Product Information
Format
Affinity Purified
Presentation
Purified Rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Amyloid-like protein 1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Amyloid-like protein 1.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Amyloid-like protein 1 in human cerebral cortex and thalamic tissues.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the extracellular domain of Human Amyloid-like protein 1.
Epitope
Extracellular domain
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Rat, Mouse based on 100% sequence homology. Immunogen is 94% identical to Bovine
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_005157
Gene Symbol
APLP1
Purification Method
Antigen Affinity Purified
UniProt Number
P51693
Molecular Weight
~72 kDa observed. A ~90kDa band may appear in some lysates due to glycosylation: Kim. TW., et al. (1995). Brain Res Mol Brain Res. 32(1):36-44.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Human Cortex tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Amyloid-like protein 1 in 10 µg of Human Cortex tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
