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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN45 |
| Description | Anti-Myelin Regulatory Factor (MRF) Antibody |
| Alternate Names |
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| Background Information | Myelin Regulatory Factor (MRF), also known as Myelin regulatory factor, MYRF, and encoded by the gene MYRF/C11orf9/KIAA0954/MRF, is an interesting protein that gets cleaved into two chains. The N terminal component specifically activates transcription of myelin gene in the CNS oligodendrocytes and pre-oligodendrocytes. The C-terminal portion, which is remains membrane bound, appears to play no role in gene induction but its complete role is still being discovered. The N-terminal portion of MRF activates MBP, MOG, MAG, and PLP among other genes, but it is not required for oligodendrocyte differentiation but is required for the maintenance of myelin in the adult. MRF whole factor is localized to the endoplasmic reticulum. The cleaved N terminal portion translocates to the nucleus and the C-terminal portion remains in the endoplasmic reticulum. Interestingly MRF can be found expressed outside of oligodendrocytes and oligo precursors in the brain including regions such as the lung, basal ganglion and liver. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Myelin Regulatory Factor (MRF) Antibody is validated for use in western blotting & IHC for the detection of Myelin Regulatory Factor (MRF). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Myelin Regulatory Factor (MRF) in 10 µg of ARPE19 cell lysate. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Myelin Regulatory Factor (MRF) in human cerebral cortex, human retina, and human cerebellum tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Myelin Regulatory Factor (MRF). |
| Epitope | cytoplasmic domain, N-terminal chain |
| Concentration | 1.0 mg/mL |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat, Hamster, Horse, Monkey based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~160 kDa observed. The expected molecular weight is 125 kDa and ~160kDa when glycosylated. The protein was cleaved into a ~90 kDa N-terminal fragment from the full length of ~160 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myelin Regulatory Factor (MRF) in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |