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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN458 |
| Replaces | AB5960;AB5966 |
| Description | Anti-Choline Transporter Antibody |
| Alternate Names |
|
| Background Information | High affinity choline transporter 1 (UniProt: Q9GZV3; also known as Hemicholinium-3-sensitive choline transporter, CHT, Solute carrier family 5 member 7) is encoded by the SLC5A7 (also known as CHT1) gene (Gene ID: 60482) in human. CHT is a multi-pass membrane protein with six extracellular domains, eleven transmembrane domains, and six cytoplasmic domains. It is expressed in cholinergic neurons in putamen, spinal cord, and medulla and serves as a transmembrane transporter that imports choline from the extracellular space into neurons with high affinity in a sodium and chloride ion-dependent manner. In neurons it is acetylated by the action of choline acetyltransferase to generate acetylcholine. Choline uptake is shown to be the rate-limiting step in acetylcholine synthesis. The majority of CHT is localized intracellularly including a proportion present on acetylcholine-containing synaptic vesicles. Diminished levels of acetylcholine in selected areas of the nervous system are linked to several neurodegenerative diseases. Mutation in SLC5A7 gene have been linked to neuronopathy and myasthenic syndrome that is characterized by failure of neuromuscular transmission, fatigability, and muscle atrophy and weakness. (Ref.: Bauche, S., et al. (2016). Am. J. Hum. Genet. 99(3); 753-761; Barwick, KES., et al. (2012). Am. J. Hum. Genet. 91(6); 1103-1107). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Choline Transporter, Cat. No. ABN458, is a rabbit polyclonal antibody that detects Choline Transporter and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Choline Transporter in rat spinal cord and human cerebral cortex tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 77 amino acids from the C-terminal region of human High affinity choline transporter. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects high affinity choline transporter. It targets an epitope within the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Rat. Predicted to react with Human, Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~63 kDa observed; 63.2 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Rat spinal cord tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Choline Transporter in Rat spinal cord tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at +2°C to +8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |