Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN904-25UG |
| Description | Anti-GAD 65/67 |
| Alternate Names |
|
| Background Information | Glutamate decarboxylase 2/Glutamate decarboxylase 1 (UniProt: Q05329/Q99259; also known as 65 kDa/67kDa glutamic acid decarboxylase, GAD-65/67, Glutamate decarboxylase 65/67 kDa isoform) is encoded by GAD2/GAD1 (also known as GAD65/GAD67) gene (Gene ID: 2572/2571) in human. Glutamate decarboxylase isoforms are expressed in the brain where it is involved in gamma-aminobutyric acid (GABA) synthesis. Glutamate decarboxylase 2 is also expressed in pancreatic tissue. Both GAD65 and GAD67 synthesize GABA at different locations in the cell and at different developmental stages. Usually, GAD1 is distributed evenly throughout the cell while isoform GAD2 is mainly localized to nerve terminals. GAD1 synthesizes GABA for neuronal activity that is related to as synaptogenesis and protection from neural injury. However, GAD2 isoform synthesizes GABA for neurotransmission activity. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-GAD 65/67 Antibody, Cat. No. ABN904, is a highly specific rabbit polyclonal antibody that targets Human, Mouse, and Rat GAD2 and GAD1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GAD 65/67 in human cerebellum, mouse cerebral cortex, and rat cerebral cortex tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human glutamate decarboxylase 2 (GAD2). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects GAD2 and GAD1 in human, Mouse, and Rat. It targets an epitope within 14 amino acids from the C-terminal region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| Molecular Weight | ~60/62kDa observed; 65.41/66.90 kDa calculated, respectively for GAD2 and GAD1. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human brain tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected GAD 65/67 in 1 0 µg of human brain tissue lysate . |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |