Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H | IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN910-25UG |
| Description | Anti-Aquaporin 4 |
| Alternate Names |
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| Background Information | Aquaporin-4 (UniProt: P47863; also known as AQP-4, Mercurial-insensitive water channel, MIWC) is encoded by the Aqp4 gene (Gene ID: 25293) in rat. Aquaporin-4 is a multi-pass membrane homotetrameric protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Aquaporin 4, Cat. No. ABN910, is a highly specific rabbit polyclonal antibody that targets Aquaporin 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue. Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in mouse cerebral cortex tissue. Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution of this antibody detected Aquaporin 4 in mouse cerebral cortex tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |