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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | ICC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1047 |
| Description | Anti-phospho AKAP-12/Gravin Antibody (Thr766) |
| Alternate Names |
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| Background Information | A-kinase anchor protein 12 (UniProt Q02952; also known as A-kinase anchor protein 250 kDa, AKAP-12, AKAP 250, Gravin, Myasthenia gravis autoantigen, SSeCKS) is encoded by the AKAP12 (also known as AKAP250) gene (Gene ID 9590) in human. AKAP-12/Gravin is a member of the AKAPs (A-kinase anchoring proteins) family of scaffold proteins that are characterized by a binding domain for the RI/RII regulatory subunit of protein kinase A (a.a. 1541-1554 of AKAP-12) and play critical roles in establishing spatial constraints for multivalent signalling assemblies. AKAP-12/Gravin form a scaffold with PKA and the mitotic kinases, Aurora A and Plk1, at the mother spindle pole during metaphase, guarding against errors occurring during cell division by constraining active Aurora A and Plk1 at the end of the spindle to facilitate signal relay from one kinase to the next. AKAP-12/Gravin is phosphorylated on Thr766 in mitotic cells, T766A phosphosite mutant is unable to interact with Plk1 and negatively impacts cell proliferation. AKAP-12/Gravin is reported to be depleted in proliferating germ line-derived tumors from patients with testicular seminoma, and seminiferous tubular cells divide more rapidly in Akap12-knockout mice. Mutation or amplification of AKAP12 has also been linked to melanoma, prostate, and ovarian cancers in human. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antibody serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-phospho AKAP-12/Gravin Antibody (Thr766) is validated for use in Immunocytochemistry, Western Blotting for the detection of phospho AKAP-12/Gravin. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected an asymmetric enrichment of AKAP-12/Gravin pThr766 immunoreactivity at the mother spindle poles over the daughter spindle poles by fluorescent immunocytochemistry staining of fixed metaphase MEFs and prometaphase HEK293 cells (Hehnly, H., et Al. (2015). Elife. 4. pii: e09384). Immunocytochemistry Analysis: A representative lot detected AKAP-12/Gravin Thr766 phosphorylation in mitotic HEK293 and RAT-2 cells (from prophase through telophase/cytokinesis), but not cells in interphase, by fluorescent immunocytochemistry staining of methanol-fixed and permeabilized cells (Canton, D.A., et Al. (2012). Mol.Cell. 48(4):547-559). Western Blotting Analysis: A representative lot detected AKAP-12/Gravin Thr766 phosphorylation in HEK293 cell lysate (Hehnly, H., et Al. (2015). Elife. 4. pii: e09384). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a human AKAP-12/Gravin sequence containing phosphorylated Thr766. |
| Epitope | pThr766. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | The Thr766 numbering is based on the mature sequence (Met1 removed) of human Gravin isoform 1 (Alpha), which is equivalent to Thr668 of isoform 2 (Beta) and Thr661 of isoform 3 (Gamma). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. Predicted to react with Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Unpurified. |
| UniProt Number |
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| Molecular Weight | 191.4 kDa (isoform 1; Alpha), 181.6 kDa (isoform 2; Beta), 180.9 kDa (isoform 3; Gamma) calculated (Met1 removed). Due to posttranslational phosphorylation on multiple sites, target band size of greater than 250 kDa was reported (Hehnly, H., et Al. (2015). Elife. 4. pii: e09384; Canton, D.A., et Al. (2012). Mol.Cell. 48(4):547-559). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected AKAP-12/Gravin Thr766 phosphorylation in HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |