Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | FC, IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1484-25UL |
| Description | Anti-RASSF1 |
| Alternate Names |
|
| Background Information | Ras association domain-containing protein 1 (UniProt: Q9NS23; RASSF1) is encoded by the RASSF1 (also known as RDA32) gene (Gene ID: 11186) in human. RASSF1 serves as a potential tumor suppressor and is shown to be essential for death receptor-dependent apoptosis. It mediates the activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. In association with MOAP1, it promotes Bax conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Eight isoforms of RASSF1 have been described that are produced by alternative promoter usage and alternative splicing. The immunogen sequence used to generate this antibody is conserved in isoforms A, D, E, F and g. Isoform A is ubiquitously expressed and is shown to localize to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Isoform A is reported to interact with CDC20, an activator of the anaphase-promoting complex (APC), resulting in the inhibition of APC activity and mitotic progression. It Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. It also disrupts interactions among MDM2, DAXX and USP7 and thereby contributes to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-RASSF1, Cat. No. ABS1484, is a rabbit polyclonal antibody tht detects Ras association domain-containing protein 1 in human and rat tissues and has been tested for use in Flow Cytometry, Immunohistochemistry (paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 1 µg from a representative lot detected RASSF1 in Jurkat cells. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RASSF1 in human ovarian cancer, human placenta, and human pancreas tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Ras association domain-containing protein 1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Ras association domain-containing protein 1 in human and rat tissues. It targets an epitope within 14 amino acids from the N-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~39 kDa observed; 39.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat liver tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected RASSF1 in 10 µg of r at liver tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |