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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IHC, Neutralization Assay | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1593 |
| Description | Anti-sFRP-1 Antibody |
| Alternate Names |
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| Background Information | Secreted frizzled-related protein 1 (UniProt Q8N474; also known as FRP-1, SARP-2, Secreted apoptosis-related protein 2, sFRP-1) is encoded by the SFRP1 (also known as FRP, FRP1, SARP2) gene (Gene ID 6422) in human. Canonical Wnt signaling is constitutively suppressed in the absence of Wnt, and its activation in the presence of Wnt can be blocked by secreted inhibitors, including the dickkopf-related proteins (DKK) and the secreted frizzled-related proteins (sFRP1-5). sFRPs share homology with the FZD receptor and compete againt FZD for Wnt binding in the extracellular space, sFRPs may also exert their effects by interacting with each other and FZD receptors. sFRP-1 is present during both cardiac development and adult life and is abundantly expressed in the mouse and human heart. Study conducted with Sfrp1-knockout mice indicates a role of sFRP-1 in age-related cardiac deterioration and fibrosis. Human sFRP-1 is produced with a signal peptide sequence (a.a. 1-31), the removal of which yields the mature protein (a.a. 32-314) with an N-terminal FZ (a.a. 53-169) domain, followed by a netrin (NTR) domain (a.a. 186-306). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect sFRP-1 using this rabbit polyclonal Anti-sFRP-1, Cat. No. ABS1593, validated for use in Immunohistochemistry, Neutralization, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:260 dilution from a representative lot detected sFRP-1 in human kideny and testis tissue sections (Courtesy of Jeffrey Rubin, M.D., Ph.D., and Aykut Üren, M.D., Ph.D.). Neutralizing Analysis: A representative lot, in combination with recombinant Wtn5a and an anti-DKK1 antibody, increased migration and mammosphere formation of EpCAM-positive (EPC+) human mammary epithelial cells (MECs) (Scheel, C., et al. (2011). Cell. 145(6):926 940). Neutralizing Analysis: A representative lot enhanced the formation of TRACP+ mutinucleated and mononucleated cells from co-cultured murine splenic and primary osteoblastic cells upon PGE2 and 1 ,25 (OH)2D3 stimulatation (Häusler, K.D., et al. (2004). J. Bone Miner. Res. 19(11):1873-1881). Note: Detection of endogenous sFRP-1 in whole cell lysates or tissue samples is difficult without an enrichment step prior to immunoblotting (e.g. by immunoprecipitation or heparin affinity chromatography). |
| Biological Information | |
|---|---|
| Immunogen | Mammalian expressed human sFRP-1, purified from condition culture medium (Häusler, K.D., et al. (2004). J. Bone Miner. Res. 19(11):1873-1881; Uren, A., et al. (2000). J. Biol. Chem. 275(6):4374-4382). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody neutralized the inhibitory activity of sFRP-1 (Scheel, C., et al. (2011). Cell. 145(6):926 940; Häusler, K.D., et al. (2004). J. Bone Miner. Res. 19(11):1873-1881). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A purified. |
| UniProt Number |
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| Molecular Weight | ~39 kDa observed. Target band size appears larger than the calculated molecular weights of 32.54/35.39 kDa (mature/pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting of human sFRP-1 recombinant protein. Western Blotting Analysis: 1 µg/mL of this antibody detected in 10 ng of mammalian expressed, secreted human sFRP-1. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |