Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1633 |
| Description | Anti-IST1 Homolog |
| Alternate Names |
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| Background Information | IST1 homolog (UniProt: P53990; also known as hIST1, Putative MAPK-activating protein PM28) is encoded by the IST1 (also known as KIAA0174) gene (Gene ID: 9798) in human. IST1 is an ESCRT-III-like protein that is involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis. IST1 is also involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, it is involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. It is shown to regulate early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST. Six isoforms of IST1 homolog have been described that are generated by alternative splicing. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-IST1 Homolog, Cat. No. ABS1633,is a rabbit polyclonal antibody that detects IST1 homolog and has been tested for use in Immunofluorescence and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected IST1 Homolog in 10 µg of HeLa cell lysate. Western Blotting Analysis: A representative lot detected IST1 Homolog in Western Blotting applications (Bajorek, M., et. al. (2009). Nat Struct Mol Biol. 16(7):754-62; Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73). Immunofluorescence Analysis: A representative lot detected IST1 Homolog in Immunofluorescence applications (Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73). |
| Biological Information | |
|---|---|
| Immunogen | A recombinant fragment corresponding to the first 189 amino acids from the N-terminal half of human IST1 homolog. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects IST1 homolog in human cells. It targets an epitope within the first 189 amino acids from the N-terminal half. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed; 39.75 kDa calculated.. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected IST1 Homolog in 10 µg of Raji cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |