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Millipore Anti-Ist1 Homolog, Cat. No. Abs1633,Is A Rabbit Polyclonal Antibody That Detects Ist1 Homolog And Has Been Tested For Use In Immunofluorescence And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
IST1 homolog (UniProt: P53990; also known as hIST1, Putative MAPK-activating protein PM28) is encoded by the IST1 (also known as KIAA0174) gene (Gene ID: 9798) in human. IST1 is an ESCRT-III-like protein that is involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis. IST1 is also involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, it is involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. It is shown to regulate early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST. Six isoforms of IST1 homolog have been described that are generated by alternative splicing.
Product Information
Format
Serum
Presentation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Applications
Application
Anti-IST1 Homolog, Cat. No. ABS1633,is a rabbit polyclonal antibody that detects IST1 homolog and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
Immunofluorescence
Western Blotting
Application Notes
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected IST1 Homolog in 10 µg of HeLa cell lysate. Western Blotting Analysis: A representative lot detected IST1 Homolog in Western Blotting applications (Bajorek, M., et. al. (2009). Nat Struct Mol Biol. 16(7):754-62; Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73). Immunofluorescence Analysis: A representative lot detected IST1 Homolog in Immunofluorescence applications (Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73).
Biological Information
Immunogen
A recombinant fragment corresponding to the first 189 amino acids from the N-terminal half of human IST1 homolog.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects IST1 homolog in human cells. It targets an epitope within the first 189 amino acids from the N-terminal half.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Chimpanzee based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Gene Symbol
IST1
KIAA0174
Purification Method
Affinity Purified
UniProt Number
P53990
Molecular Weight
~50 kDa observed; 39.75 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected IST1 Homolog in 10 µg of Raji cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
