Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, IP, WB | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1644-25UL |
| Description | Anti-DsbA-L |
| Alternate Names |
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| Background Information | Glutathione S-transferase kappa 1 (UniProt: Q9DCM2; also known as EC:2.5.1.18, GST 13-13, GST class-kappa, GSTK1-1, mGSTK1, Glutathione S-transferase subunit 13, DsbA-L) is encoded by the Gstk1 gene (Gene ID: 76263) in murine species. DsbA-L is a disulfide bond A oxidoreductase like homodimeric mitochondrial protein that is predominantly expressed in heart, kidney, liver and skeletal muscle. It is also highly expressed in adipose tissue and its expression level is negatively correlated with obesity in mice and humans. Overexpression of DsbA-L is shown to promote adiponectin multimerization. Suppression of DsbA-L expression can selectively reduce adiponectin levels and secretion in 3T3-L1 adipocytes. Expression of DsbA-L in 3T3-L1 adipocytes is shown to be stimulated by the insulin sensitizer rosiglitazone and inhibited by TNFalpha. Adiponectin expression is reduced in both human obesity and mouse models of genetic obesity. DsbA-L is also shown to co-localize with the endoplasmic reticulum (ER) marker protein disulfide isomerase and it interacts with the ER chaperone protein Ero1-L in 3T3-L1 adipocytes. Deletion of the first 6 amino acid residues at the N terminus greatly impaired DsbA-L localization in the ER. (Ref.: Liu, M et Al. (2015). J Biol Chem. 290(16):10143-8). |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol. |
| Applications | |
|---|---|
| Application | Anti-DsbA-L, Cat. No. ABS1644, is a rabbit polyclonal antibody that detects Glutathione S-transferase kappa 1 (DsbA-L) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected DsbA-L in A431, HUVEC, NIH/3T3 cells. Western Blotting Analysis: A representative lot detected DsbA-L in mouse brain, mouse kidney, and mouse liver (Liu, M., et. Al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. Al. (2015). J Biol Chem. 290(16):10143-8). Immunoprecipitation Analysis: A representative lot detected DsbA-L in 3T3-L1 adipocytes (Liu, M., et. Al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. Al. (2015). J Biol Chem. 290(16):10143-8). Immunocytochemistry Analysis: A representative lot detected DsbA-L in adipocytes on differentiation day 7 were used for the following studies. confocal immunofluorescence study of DsbA-L co-localization with the mitochondrial marker (MitoTracker) and the ER marker (PDI) in 3T3-L1 adipocytes (Lui, M., et. Al. (2015). J Biol Chem. 290(16):10143-8). |
| Biological Information | |
|---|---|
| Immunogen | Myc-tagged full length recombinant protein from mouse. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects DsbA-L (Glutathione S-transferase kappa 1) in human and mouse cells. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | 28 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse kidney tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DsbA-L in 10 µg of mouse kidney tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |