Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS2053 |
| Description | Anti-Glutaredoxin-2 (Grx2) |
| Alternate Names |
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| Background Information | Glutaredoxin-2, mitochondrial (UniProt: Q9NS18) is encoded by the GLRX2 (also known as GRX2, CGI-133) gene (Gene ID: 51022) in human. Glutaredoxin-2 is a glutathione-dependent oxidoreductase that is widely expressed and facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Its active form is a monomer and it can dimerize to an inactive form where homodimers are linked by one 2Fe-2S cluster. The 2Fe-2S may serve as a redox sensor and the presence of one-electron oxidants or reductants can lead to the loss of the 2Fe-2S cluster and subsequent monomerization and activation of the enzyme. Glutaredoxin-2 acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. It can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Glutaredoxin-2 is known to efficiently catalyze both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression of glutaredoxin-2 decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Glutaredoxin-2 (Grx2), Cat. No. ABS2053, is a rabbit polyclonal antibody that detects Glutaredoxin-2, mitochondrial, and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glutaredoxin-2 (Grx2) in human cerebellum and human tonsil tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Glutaredoxin-2 . |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects mitochondrial isoform of glutatedoxin-2. It targets an epitope with in 16 amino acids from the C-terminal half. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Chimpanzee, Baboon, Monkey based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed; 18.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A549 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Glutaredoxin-2 (Grx2) in 10 µg of A549 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |