Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IF, WB | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS2087 |
| Description | Anti-Mff |
| Alternate Names |
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| Background Information | Mitochondrial fission factor (UniProt: Q9GZY8; also known as Mff) is encoded by the MFF (also known as C2orf33, AD030, AD033, GL004) gene (Gene ID: 56947) in human. Mff is a single-pass type IV membrane protein that is highly expressed in heart, kidney, liver, brain, muscle, and stomach. Mff is a homodimeric protein, but may also interact with other proteins. It plays a role in mitochondrial and peroxisomal fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. It is also suggested to be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles. Mff has a cytoplasmic domain (aa 1-322), a helical domain (aa 323-340), and a mitochondrial intermembrane domain (aa 341-342). Mff is shown to be an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells and its down-regulation inhibits mitochondrial recruitment of Drp1 and induces mitochondria elongation. Also, exogenous expression of Mff is shown to induce extensive mitochondrial fission. Mutations in MFF gene can cause a form of encephalopathy that is characterized by delayed psychomotor development, severe hypotonia with inability to walk, and microcephaly. (Ref.: Otera, H., et al. (2010). J. Cell Biol. 191 (6); 1141 1158; Gandre-Babbe, S., and van der Bliek, AM. (2008). Mol. Biol. Cell. 19(6): 2402-2412). |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Mff, Cat. No. ABS2087, is a rabbit polyclonal antibody that detects Mitochondrial fission factor (Mff) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Mff in Western Blotting applications (Mishra, P., et. al. (2015). Cell Metab. 22(6):1033-44). Immunofluorescence Analysis: A representative lot detected Mff in Immunofluorescence applications (Mishra, P., et. al. (2015). Cell Metab. 22(6):1033-44). |
| Biological Information | |
|---|---|
| Immunogen | His-taged full length human recombinant mitochondrial fission factor (Mff). |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects mitochondrial fission factor (Mff) in human cells. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | 38.47 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells with Mitotracker (TM). Immunocytochemistry Analysis: A 1:2,000 dilution of this antibody detected Mff in HeLa cells with Mitotracker (TM). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |