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Millipore Anti-Icdh, Cat. No. Abs2090, Is A Rabbit Polyclonal Antibody That Detects Isocitrate Dehydrogenase [Nadp]. It Has Been Tested For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Isocitrate dehydrogenase [NADP] (also known as IDH, ICDH) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. In eukaryotes there are at least three isozymes of IDH: two are located in the mitochondrial matrix (one NAD+-dependent, the other NADP+-dependent), while the third one (also NADP+-dependent) is cytoplasmic. In E.coli the activity of a NADP+-dependent ICDH is controlled by the phosphorylation of a serine residue and the phosphorylated form of ICDH is completely inactivated. It has been reported that if Ser113 is replaced by alanine, cysteine, threonine, or tyrosine there is some loss of enzymatic activity. However, substitution by aspartic acid completely abolishes its activity. ICDH contains three domains: a large alpha + beta domain, a small alpha/beta domain and an alpha/beta clasp-like domain involving both subunits. (Ref.: Hurley, JH et al (1989). Proc. Natl. Acad. Sci. USA. 86 (22); 8635-8639).
Product Information
Format
Serum
Presentation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Applications
Application
Anti-ICDH, Cat. No. ABS2090, is a rabbit polyclonal antibody that detects Isocitrate dehydrogenase [NADP]. It has been tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected ICDH in 20 UL Legionella cell lysate. Western Blotting Analysis: A representative lot detected ICDH in E. Coli cell lysate (Xu, L., et. al. (2010). PLoS Pathog. 6(3):e1000822; Lu, C., et. al. (2016). Sci Signal. 9(412):ra11).
Biological Information
Immunogen
His-tagged full length recombinant isocitrate dehydrogenase from Legionella pneumophila, strain Lp02.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This polyclonal antibody detects isocitrate dehydrogenase in E. coli and Legionella pneumophila.
Isotype
IgG
Species Reactivity
E. coli
Species Reactivity Note
E. Coli, Legionella pneumophila.
Antibody Type
Polyclonal Antibody
Gene Symbol
ICDH
Purification Method
Unpurified
UniProt Number
No verified Uniprot
PUBMED 25977442
Molecular Weight
45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in E.Coli cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ICDH in 20 UL E.Coli cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
