Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS2099 |
| Description | Anti-STRN3 |
| Alternate Names |
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| Background Information | Striatin-3 (UniProt: Q13033; also known as Cell cycle autoantigen SG2NA, S/G2 antigen) is encoded by the STRN3 (also known as GS2NA, SG2NA) gene (Gene ID: 29966) in human. Striatin-3 is a calmodulin-binding protein that bonds calmodulin a calcium dependent manner and may function as scaffolding or signaling protein. Two isoforms of Striatin-3 have been reported that are produced by alternative splicing. Its calmoduln-binding region is located within amino acids 166-183. It also contains a caveolin-binding region (aa 71-79), six WD repeats, and a coiled coil region (aa 77-136). The coiled coil domain and the PP2A subunit can form a stable core complex with a 2:2 stoichiometry. The coiled coil domain of striatin-3 is a novel type of PP2A regulatory subunit that functions as a scaffold for the assembly of the STRIPAK complex. PP2A and certain kinases, such as germinal central kinase III (GCKIII) interact with striatin to form striatin-interacting phosphatase and kinase (STRIPAK) complex that are known to regulate multiple cellular events. Wild-type striatin-3 is shown to strongly suppress apoptosis in Jurkat cells induced by the GCKIII kinase MST3. However, mutant forms of striatin-3 fail to exhibit this effect. (Ref.: Lu, Q., et al. (2004). Proc. Natl. Acad. Sci. USA 101(49); 17126-17131; Chen, C., et al. (2014). J. Biol. Chem. 289(14); 9651-61). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-STRN3, Cat. No. ABS2099, is a rabbit polyclonal antibody that detects Striatin-3 and has been tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected STRN3 in HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Striatin-3. The immunogen sequence is conserved in both alpha and beta isoforms. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detect Striatin-3 in human and murine cells. It targets an epitope within 17 amino acids from the N-terminal half. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~87 kDa observed; 87.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH3T3 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected STRN3 in 10 µg of NIH3T3 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |