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Millipore Anti-Hmg-Lysine, Cat. No. Abs2108, Is A Rabbit Polyclonal Antibody That Detects Hydroxymethylglutaryl-Lysine Conjugated Proteins And Has Been Tested For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Acetylation of lysine residues in proteins has emerged as an important mechanism in regulation and coordination of normal and pathophysiological cell metabolism. Lysine HMGylation is a newer post-translational protein modification that occurs under normal basal conditions. In murine models of HMG-CoA lyase deficiency, higher levels of protein lysine HMGylation has been observed. This rabbit polyclonal antibody specifically detects chemically modified proteins containing hydroxymethylglutaryl-lysine modifications, but not unmodified proteins. (Ref.: Wagner, GR et al. (2017). Cell Metabolism 25(4); 823-837).
Product Information
Format
Purified
Presentation
Purified rabbit polyclonal antibody in PBS with 0.1% sodium azide.
Applications
Application
Anti-HMG-Lysine, Cat. No. ABS2108, is a rabbit polyclonal antibody that detects hydroxymethylglutaryl-lysine conjugated proteins and has been tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected HMG-Lysine in Western Blotting applications (Wagner, G.R., et. al. (2017). Cell Metab. 25(4):823-837; Anderson, K.A., et. al. (2017). Cell Metab. 25(4):838-855.e15).
Western Blotting Analysis: A 1:500 dilution from a representative lot detected HMG-Lysine in HMG-BSA conjugate (Courtesy of Dr Kristin Anderson from Duke University).
Biological Information
Immunogen
3-hydroxy-3-methyl-glutaryl (HMG)-Lysine conjugated to BSA.
Epitope
unknown
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects BSA-conjugated 3-hydroxy-3-methyl-glutaryl (HMG)-Lysine. It recognizes BSA chemically modified to contain HMG-lysine modifications, but not unmodified BSA.
Isotype
IgG
Species Reactivity
All
Species Reactivity Note
Predicted to react with all species.
Antibody Type
Polyclonal Antibody
Purification Method
Affinity Purified
Molecular Weight
~75 kDa observed (BSA conjugate). Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting with HMG-BSA conjugate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected HMG-Lysine in HMG-Lysine-BSA conjugate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
