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Millipore Anti-Ppox, Cat. No. Abs2123, Is A Rabbit Polyclonal Antibody That Detects Protoporphyrinogen Oxidase And Has Been Tested For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Protoporphyrinogen oxidase (UniProt: P50336; also known as EC: 1.3.3.4, PPO, PPOX) is encoded by the PPOX gene (Gene ID: 5498) in human. Protoporphyrinogen oxidase is a mitochondrial inner membrane enzyme that catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. It is involved in step one of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX. It is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas and may be present as a monomer or active homodimeric form. Defects in PPOX are linked to variegate porphyria that lead to severe deficiency of this enzyme that result in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver.
Product Information
Format
Unpurified
Presentation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-PPOX, Cat. No. ABS2123, is a rabbit polyclonal antibody that detects Protoporphyrinogen oxidase and has been tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected PPOX in undifferentiated (day 0) and maturing (day 3 or 4) mouse erythroleukemia (MEL) cells (Chung, J., et. al. (2015). Sci Signal. 8(372):ra34). and differentiated Mouse erythroleukemia (MEL) cells (Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).
Biological Information
Immunogen
Full length recombinant human Protoporphyrinogen oxidase (PPOX).
Epitope
unknown
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects Protoporphyrinogen oxidase (PPO) in Human and Mouse cells.
Isotype
IgG
Species Reactivity
Mouse
Species Reactivity Note
Mouse. Predicted to react with Human based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_000300
Gene Symbol
PPOX
Purification Method
Unpurified
UniProt Number
P50336
Molecular Weight
~53 kDa observed; 50.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in lystate from mitochondria isolated from differentiated DS19 cells.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PPOX in lystate from mitochondria isolated from differentiated DS19 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
