Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS2124-25UL |
| Description | Anti-Ferrochelatase |
| Alternate Names |
|
| Background Information | Ferrochelatase, mitochondrial (UniProt: P22830; also known as EC: 4.99.1.1, Heme synthase, Protoheme ferro-lyase) is encoded by the FECH gene (Gene ID: 2235) in human. Ferrochelatase catalyzes the terminal reaction of the heme biosynthetic pathway in all cells. It catalyzes the insertion of ferrous iron into protoporphyrin IX, yielding heme. In eukaryotic cells, it is located in the inner mitochondrial membrane with its active site facing the mitochondrial matrix. Human ferrochelatase is a homodimer with each subunit composed of five regions: a mitochondrial localization sequence, the N terminal domain, two folded domains, and a C terminal extension. The active pocket of ferrochelatase consists of two hydrophobic "lips" and a hydrophilic interior. The hydrophobic lips face the inner mitochondrial membrane and facilitate the passage of the poorly soluble protoporphyrin IX substrate and the heme product via the membrane. Reduced levels of ferrochelatase activity are observed in patients with protoporphyria. Point-mutations in the FECH gene are reported to be associated with certain cases of erythropoietic protoporphyria. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Ferrochelatase, Cat. No. ABS2124, is a rabbit polyclonal antibody that detects Ferrochelatase, mitochondrial. It has been tested for use in Immunoprecipitation and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot detected Ferrochelatase in differentiated mouse erythroleukemia (MEL) cells (Karr, S.R., et. al. (1988). Biochem J. 254(3):799-803). Western Blotting Analysis: A representative lot detected Ferrochelatase in differentiated mouse erythroleukemia (MEL) cells and transfected heterologous cells. (Chen, W., et. al. (2010). Blood. 116(4):628-30; Anand, S., et. al. (2009). Clin Cancer Res. 15(10):3333-43; Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896). |
| Biological Information | |
|---|---|
| Immunogen | Flag-tagged recombinant fragment corresponding to the mature mitochondrial form containing 369 amino acids (aa 55-423) from human Ferrochelatase. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects ferrochelatase in human and murine species. It targets the mature form of ferrochelatase. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~43 kDa observed; 47.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ferrochelatase in 10 µg of K562 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |