Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS2131 |
| Description | Anti-phospho Rab1 (Thr75) |
| Alternate Names |
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| Background Information | Ras-related protein Rab-1A (UniProt: P62820; also known as YPT1-related protein) is encoded by the RAB1A (also known as RAB1) gene (Gene ID: 5861) in human. Rab1 is a member of the small GTPase superfamily. Rab proteins are the largest family of small Ras-like GTPases. Three isoforms of Rab1 have been reported that are produced by alternative splicing. Rab GTPases are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. They cycle between an inactive GDP-bound form and an active GTP-bound form, which is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering, and fusion. Rab1 is reported to regulate vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface. Rab1 is also shown to be involved in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Rab1 is shown to be phosphorylated at Thr75 by TGF-beta activated kinase 1 (TAK1), which preferentially phosphorylates the inactive (GDP-bound) state of Rab1. Phosphorylation disrupts interaction with GDP dissociation inhibitor 1 (GDI1), but not GEF or GAP. Phosphorylation at Thr75 is shown to be essential for Rab1 function. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho Rab1 (Thr75), Cat. No. ABS2131, is a rabbit polyclonal antibody that detects Ras-related protein Rab-1A and has been tested for use in Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot detected phospho Rab1 (Thr75) in Western Blotting applications (Levin, R.S., et. al. (2016). Proc Natl Acad Sci USA. 113(33):E4776-83). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 14 amino acids surrounding phosphorylated Thr75 from the N-terminal half of human Ras-related protein Rab-1A. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Ras-related protein Rab-1A (Rab1) in human cells. It targets an epitope within 14 amino acids surrounding phosphorylated Thr75. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Bovine, Mouse, Rat, Porcine, Sheep based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~48 kDa observed; 22.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in lysate from HEK293T stably expressing GST-Rab1a-Tak1. Western Blotting Analysis: A 1:250 dilution of this antibody detected phospho Rab1 (Thr75) in lysates from HEK-293T stably expressing GST-Rab1a-Tak1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |