| Description | |
|---|---|
| Catalogue Number | ABS2183 |
| Description | Anti-Coagulation factor VIIa |
| Alternate Names |
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| Background Information | Coagulation factor VII (UniProt: P08709; also known as EC: 3.4.21.21, Proconvertin, Serum prothrombin conversion accelerator, SPCA) is encoded by the F7 gene (Gene ID: 2155) in human. Coagulation factor VII is a member of the peptidase S1 family that initiates the extrinsic pathway of blood coagulation. It is serine protease that is synthesized with a signal peptide (aa 1-20) and a propeptide (aa 21-60) and circulates in the blood in a zymogen form. The mature form is a heterodimer of a light and a heavy chain. It is a vitamin K-dependent protease that binds to calcium following enzymatic carboxylation of some glutamate residues. Factor VII is converted to Factor VIIa by the action of Factor Xa, Factor XIIa, Factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, Factor VIIa converts factor X to factor Xa by cleavage of Arg-|-Ile bond. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium. Factor VII deficiency resulting from mutations in F7 gene can lead to hemorrhagic complications. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Coagulation factor VIIa Antibody, Cat. No. ABS2183, is a rabbit polyclonal antibody that detects Coagulation factor VIIa and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting. |
| Key Applications |
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| Application Notes | Immunofluorescence Analysis: A representative lot detected Coagulation factor VIIa in endothelial cell cultures incubated with rFVIIa (6 mg/mL) for 2 h at 37˚C. Immunocytochemistry Analysis: A representative lot detected Coagulation factor VIIa in umbilical cord veins. |
| Biological Information | |
|---|---|
| Immunogen | Full-length human recombinant Coagulation factor VIIa. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects coagulation factor VIIa in human. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with other primates based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~57 kDa observed; 51.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human placental tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Coagulation factor VIIa in 10 µg of human placental tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |