Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS2203-25UG |
| Description | Anti-UBE2A |
| Alternate Names |
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| Background Information | Ubiquitin-conjugating enzyme E2 A (UniProt: P49459; also known as EC 2.3.2.23; E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A) is encoded by the UBE2A (also known as RAD6A) gene (GeneID: 7319) in human. UBE2A belongs to the ubiquitin-conjugating enzyme family and accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1, it plays a role in transcription regulation by catalyzing the mono-ubiquitination of histone H2B at Lys-120 that provides a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, and telomeric silencing. It is also a prerequisite for H3K4me and H3K79me formation. Defects in UBE2A gene have been linked to mental retardation, X-linked, syndromic, Nascimento-type mental retardation that is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-UBE2A Antibody, Cat. No. ABS2203, is a rabbit polyclonal antibody that detects UBE2A and has been tested for use Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected UBE2A in mouse kidney tissue lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected UBE2A in human pancreas, human cervical cancer, and human uterus tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Ubiquitin-conjugating enzyme E2 A (UBE2A). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Ubiquitin-conjugating enzyme E2 A (UBE2A) in HeLa cells and Mouse Kidney. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed; 17.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected UBE2A in HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |