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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS574 |
| Replaces | AB3434 |
| Description | Anti-Surfactant Protein D |
| Alternate Names |
|
| Background Information | Pulmonary surfactant-associated protein D (UniProt P35247; also known as Collectin-7, Lung surfactant protein D, PSP-D, SP-D) is encoded by the SFTPD (also known as COLEC7, PSPD, SFTP4) gene (Gene ID 6441) in human. Surfactant, produced by alveolar type II cells and airway Clara cells, resides at the air-liquid interface of the lungs and is most commonly known for its role in reducing surface tension. Surfactant is a lipoprotein complex composed of approximately 10% proteins, including surfactant protein A (SP-A), SP-B, SP-C, and SP-D. SP-A and SP-D belong to the collectin family of proteins and can modulate innate immunity. SP-D is known to enhance pulmonary clearance of pathogens, such as Pseudomonous aerginosa, Klebsiella pneumonia, respiratory syncytial virus (RSV) and Influenza virus. SP-D is also shown to modify allergic responsee in the lungs by binding common allergens, including house dust mite and pollen particles. SP-A-deficient and SP-D-deficient mice are susceptible to bleomycin-induced lung injury and display increased cellular inflammation, more severe lung fibrosis, and reduced survival. In addition to lung, SP-D is also reported to be detected in extrapulmonary tissues, such as kidney and pancreas. Serum levels of either SP-A or SP-D in human are correlated with mortality in patients with pulmonary fibrosis. Human SP-D is produced with a signal peptide sequence (a.a. 1-20), which is removed posttranslationally to yield the mature protein (a.a. 21-375) that contains a collagen-like domain (a.a. 46-222), a coiled coil region (a.a. 223-252), and a C-type lectin domain (a.a. 260-375). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Surfactant Protein D using this rabbit polyclonal Anti-Surfactant Protein D Antibody, Cat. No. ABS574, validated for use in Immunohistochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected surfactant protein D in human lung and pancreas tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence within the C-type lectin domain of human surfactant protein D. |
| Epitope | domain of: |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody targets a sequence within the C-type lectin domain of human surfactant protein D. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity purified. |
| UniProt Number |
|
| Molecular Weight | ~48 kDa observed. Target band size appears larger than the calculated molecular weights of 35.50/37.73 kDa (mature/pro-form) due to posttranslational modifications. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A549 cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected surfactant protein D in 10 µg of A549 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |