Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ICC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT1385-25UL |
| Description | Anti-Glypican-1 |
| Alternate Names |
|
| Background Information | Glypican-1 (UniProt: P35053; also known as GPC1, HSPG M12) is encoded by the Gpc1 gene (GeneID: 58920) in rat. Glypican-1 is attaches to the plasma membrane via glycosylphosphatidylinositol (GPI) anchors. It is a cell surface proteoglycan that bears heparan sulfate and may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Glypican-1 is required for proper skeletal muscle differentiation. It sequesters FGF2 in lipid rafts and blocks its binding to FGF receptors. Glypican-1 is specifically enriched on cancer-cell-derived exosomes and levels of glypican-1 positive exosomes correlate with tumor burden and the survival of pre- and post-surgical patients. Glypican-1 expression, both mRNA and protein, is upregulated in human pancreatic cancers cells and adjacent fibroblasts. (Ref.: Melo, SA et Al. 2015. Nature. 523(7559):177-82; Kleeff, J et Al. 1998. J. Clin. Invest. 102(9), 1662 1673). |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Glypican-1 Antibody, Cat. No. ABT1385 is a rabbit polyclonal antibody that detects Glypican-1 and is tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Glypican-1 in rat brain homogenates and rat C6 glioma cells treated with heparitinase (Liang, Y., et. Al. (1997). J Cell Biol. 139(4):851-64). Immunocytochemistry Analysis: A representative lot detected Glypican-1 in rat C6 glioma cells (Liang, Y., et. Al. (1997). J Cell Biol. 139(4):851-64). Immunohistochemistry Analysis: A representative lot detected Glypican-1 in rat spinal cord and brain (Liang, Y., et. Al. (1997). J Cell Biol. 139(4):851-64). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to the first 423 amino acids of rat Glypican-1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Glypican-1 in Rat C6 glioma cells. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Rat. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| Molecular Weight | 61.73 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in Rat C6 glioma cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Glypican-1 in Rat C6 glioma cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |