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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT146-25UG |
| Description | Anti-Occludin (rabbit polyclonal) |
| Alternate Names |
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| Background Information | Occludin is a 65-kDa integral plasma-membrane protein located at the tight junctions. It is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Occludin is believed to prevent the transport of macromolecules through the tight junction. Encoded by the OLCN gene, occludin spans the plasma membrane four times, forming two extracellular loops and exposing its NH2 and COOH terminus to the cytosol. This cytoplasmic tail binds directly to the ZO family of proteins, which link the protein complex to the actin cytoskeleton. Phosphorylation/dephosphorylation plays a major role in regulation of occludin and tight junctions. Expression of occludin is required for infection of liver cells by hepatitis C virus (HCV). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Occludin Antibody is validated for use in Western Blotting for the detection of Occludin. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminus of human Occludin. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antibody recognizes the N-terminus of Occludin. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Porcine, Primate, Equine, Canine, Bovine, Mouse, and Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~63 kDa observed. The calculated molecular weight is 63 kDa, however Occludin has been shown ranging from ~55 to ~65 kDa in western blots (Florian, P., et al. (2003). Biochemical and Biophysical Research Communications. 304:5-10). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human kidney tissue lysate. Western Blot Analysis: 0.2 µg/mL of this antibody detected Occludin in 10 µg of human kidney tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |