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Millipore This Anti-Occludin Antibody Is Validated For Use In Western Blotting For The Detection Of Occludin. - Mill (Additional S&H or Hazmat Fees May Apply)
Occludin is a 65-kDa integral plasma-membrane protein located at the tight junctions. It is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Occludin is believed to prevent the transport of macromolecules through the tight junction. Encoded by the OLCN gene, occludin spans the plasma membrane four times, forming two extracellular loops and exposing its NH2 and COOH terminus to the cytosol. This cytoplasmic tail binds directly to the ZO family of proteins, which link the protein complex to the actin cytoskeleton. Phosphorylation/dephosphorylation plays a major role in regulation of occludin and tight junctions. Expression of occludin is required for infection of liver cells by hepatitis C virus (HCV).
Product Information
Format
Affinity Purified
Control
Human kidney tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Occludin Antibody is validated for use in Western Blotting for the detection of Occludin.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human Occludin.
Epitope
N-terminus
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This antibody recognizes the N-terminus of Occludin.
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Porcine, Primate, Equine, Canine, Bovine, Mouse, and Rat based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_002529
Gene Symbol
OCLN
Purification Method
Affinity Purfied
UniProt Number
Q16625
Molecular Weight
~63 kDa observed. The calculated molecular weight is 63 kDa, however Occludin has been shown ranging from ~55 to ~65 kDa in western blots (Florian, P., et al. (2003). Biochemical and Biophysical Research Communications. 304:5-10).
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in human kidney tissue lysate.
Western Blot Analysis: 0.2 µg/mL of this antibody detected Occludin in 10 µg of human kidney tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
